Research Units
Genomics and Breeding Unit

This unit develops studies related to the genome of the olive tree and its applications. Specifically, the main lines of research are:

1. Olive tree genomics, with the main objective of assembling genomes of diverse varieties with high precision.
2. Genetic variability of the olive tree and its applicability, such as obtaining genetic markers to achieve genetic improvements in the olive tree.
3. Transcriptomic analysis applied to stress response, with special attention to resistance/susceptibility to verticillium wilt in olive trees.
4. Transcriptomic analysis applied to development processes.

Identification of Genetic Markers and Genes Putatively Involved in Determining Olive Fruit Weight

Moret, M.; Ramírez-Tejero, J.A.; Serrano, A.; Ramírez-Yera, E.; Cueva-López, M.D.; Belaj, A.; León, L.; Rosa, R.d.l.; Bombarely, A.; Luque, F. (2023)

Plants, 12, 155

DOI: 10.3390/plants12010155

The Association of Rpb4 with RNA Polymerase II Depends on CTD Ser5P Phosphatase Rtr1 and Influences mRNA Decay in Saccharomyces cerevisiae

Garrido-Godino, A.I. and Cuevas-Bermúdez, A. and Gutiérrez-Santiago, F. and Mota-Trujillo, M.C. and Navarro, F.

International Journal of Molecular Sciences.  Volume 23, Issue 4, 2022 

DOI: 10.3390/ijms23042002

Biogenesis of RNA Polymerases in Yeast

Garrido-Godino, A. I., Gutiérrez-Santiago, F. and Navarro, F.

Frontiers in Molecular Biosciences (2021)

DOI: 10.3389/fmolb.2021.811413

Several Isoforms for Each Subunit Shared by RNA Polymerases are Differentially Expressed in the Cultivated Olive Tree (Olea europaea L.)        

Fernández-Parras, I. and Ramírez-Tejero, J.A. and Luque, F. and Navarro, F.    

Frontiers in Molecular Biosciences. Volume 8, 2021

DOI: 10.3389/fmolb.2021.679292

Rpb4 and Puf3 imprint and post-transcriptionally control the stability of a common set of mRNAs in yeast

Garrido-Godino, A.I. and Gupta, I. and Gutiérrez-Santiago, F. and Martínez-Padilla, A.B. and Alekseenko, A. and Steinmetz, L.M. and Pérez-Ortín, J.E. and Pelechano, V. and Navarro, F.

RNA Biology. Volume 18, Issue 8, 2021, pages 1206-1220            

DOI: 10.1080/15476286.2020.1839229

Xrn1 influence on gene transcription results from the combination of general effects on elongating RNA pol II and gene-specific chromatin configuration           

Begley V., Jordán-Pla A., Peñate X., Garrido-Godino A.I., Challal D., Cuevas-Bermúdez A., Mitjavila A., Barucco M., Gutiérrez G., Singh A., Alepuz P., Navarro F., Libri D., Pérez-Ortín J.E. and Chávez S.    

RNA Biology. Volume 18, Issue 9, 2021, pages 1310-1323            

DOI: 10.1080/15476286.2020.1845504

Comparative analysis of genotyping by sequencing and whole-genome sequencing methods in diversity studies of olea europaea L.      

Friel J., Bombarely A., Fornell C.D., Luque F. and Fernández-Ocaña A.M.           

Plants. Volume 10, Issue 11, 2021

DOI: 10.3390/plants10112514

Verticillium wilt resistant and susceptible olive cultivars express a very different basal set of genes in roots

Jorge A. Ramírez-Tejero, Jaime Jiménez-Ruiz, Alicia Serrano, Angjelina Belaj, Lorenzo León, Raúl de la Rosa, Jesús Mercado-Blanco and Francisco Luque

BMC Genomics. 22, 229 (2021)

DOI: 10.1186/s12864-021-07545-x

Coupling the endophytic microbiome with the host transcriptome in olive roots

Antonio J. Fernández-González, Jorge A. Ramírez-Tejero, María Patricia Nevado-Berzosa, Francisco Luque, Manuel Fernández-López, Jesús Mercado-Blanco

Computational and Structural Biotechnology Journal. Volume 19, 2021, Pages 4777-4789

DOI: 10.1016/j.csbj.2021.08.035

Regulation of Eukaryotic RNAPs Activities by Phosphorylation

González-Jiménez A, Campos A, Navarro F, Clemente-Blanco A and Calvo O.

Frontiers in Molecular Biosciences (2021)

DOI: 10.3389/fmolb.2021.681865

Gene expression pattern in olive tree organs (Olea europaea L.)         

Ramírez-Tejero J.A., Jiménez-Ruiz J., Leyva-Pérez M.L.O., Barroso J.B. and Luque F.

Genes. Volume 11, Issue 5, 2020             

DOI: 10.3390/genes11050544

A Yeast Chromatin-enriched Fractions Purification Approach, yChEFs, from Saccharomyces cerevisiae

Cuevas-Bermúdez, A., Garrido-Godino, A. I., Gutiérrez-Santiago, F. and Navarro, F.

Bio-protocol 10(1): e3471 (2020)

DOI: 10.21769/BioProtoc.3471

Epigenetics regulation of Verticillium dahliae virulence: Does DNA methylation level play a role?

Jorge A. Ramírez-Tejero, Carmen Gómez-Lama Cabanás, Antonio Valverde-Corredor, Jesús Mercado-Blanco and Francisco Luque

International Journal of  Molecular  Science. 2020, 21(15), 5197

DOI: 10.3390/ijms21155197

Transposon Activation is a Major Driver in the Genome Evolution of Cultivated Olive Trees (Olea europaea L.)

Jaime Jiménez-Ruiz, Jorge A. Ramírez-Tejero, Noé Fernández-Pozo, María de la O Leyva-Pérez, Haidong Yan, Raúl de la Rosa, Angjelina Belaj, Eva Montes, Mª Oliva Rodríguez-Ariza, Francisco Navarro, Juan Bautista Barroso, Carmen R. Beuzón, Victoriano Valpuesta, Aureliano Bombarely, Francisco Luque

The Plant Genome. Volume13, Issue1, March 2020, e20010

DOI: 10.1002/tpg2.20010

Genome wild analysis and molecular understanding of the aquaporin diversity in olive trees (Olea europaea L.)      

Faize M., Fumanal, B., Luque F., Ramírez-Tejero J.A., Zou Z., Qiao X., Faize L., Gousset-Dupont A., Roeckel-Drevet P., Label P. and Venisse, J.-S.

International Journal of Molecular Sciences. Volume 21, Issue 11,2020, pages 1-31

DOI: 10.3390/ijms21114183

Prefoldin-like Bud27 influences the transcription of ribosomal components and ribosome biogenesis in Saccharomyces cerevisiae         

Martínez-Fernández V., Cuevas-Bermúdez A., Gutiérrez-Santiago F., Garrido-Godino A.I., Rodríguez-Galán O.,  Jordán-Pla A., Lois S., Triviño J.C.,  De La Cruz J. and Navarro F.       

RNA. Volume 26, Issue 10, 2020, pages 1360-1379          

DOI: 10.1261/rna.075507.120

The transcriptome of Verticillium dahliae responds differentially depending on the disease susceptibility level of the olive (Olea europaea L.) cultivar            

Jiménez-Ruiz J., Leyva-Pérez M.O., Cabanás C.G.-L., Barroso J.B.,  Luque F. and Mercado-Blanco, J.

Genes. Volume 10, Issue 4, 2019             

DOI: 10.3390/genes10040251

De novo assembly of Agave sisalana transcriptome in response to drought stress provides insight into the tolerance mechanisms

Sarwar M.B., Ahmad Z., Rashid B., Hassan S., Gregersen P.L., Leyva M.D.O., Nagy I Asp T. and Husnain T.               

Scientific Reports. Volume 9, Issue 1, 2019         

DOI: 10.1038/s41598-018-35891-6

The mRNA degradation factor Xrn1 regulates transcription elongation in parallel to Ccr4

Begley, Victoria; Corzo, Daniel; Jordán-Pla, Antonio,; Cuevas-Bermúdez, Abel; de Miguel, Maria; Pérez-Aguado, David; Machuca-Ostos, Mercedes; Navarro, Francisco; Chávez, María; Pérez-Ortín, José; Chavez, Sebastian

Nucleic Acids Research (2019)

DOI: 10.1093/NAR/GKZ660

Tolerance of olive (Olea europaea L.) cv. Frantoio to Verticillium dahliae relies on both basal and pathogen-induced differential transcriptomic responses

María de la O Leyva-Pérez, Jaime Jiménez-Ruiz, Carmen Gómez-Lama Cabanás, Antonio Valverde-Corredor, Juan B Barroso, Francisco Luque, Jesús Mercado-Blanco 

New Phytologist. Volume 217, Issue2, January 2018, Pages 671-686

DOI: 10.1111/nph.14833

Transcriptomic time-series analysis of early development in olive from seed to juvenile tree

Jiménez-Ruíz; Leyva-Pérez, María De La O; I. Vidoy-Mercado; A. Barceló-Muñoz; Luque, Francisco

BMC Genomics, 19(824) 2018   

DOI: 10.1186/s12864-018-5232-6

Rpb5, a subunit shared by the eukaryotic RNA polymerases cooperates with the prefoldin-like Bud27/URI

Verónica Martínez-Fernández; Francisco Navarro

AIMS Genetics. 5(1): 63-74 2018

DOI: 10.3934/genet.2018.1.63

The yeast prefoldin bud27

Martinez-Fernandez, V.; A.I. Garrido-Godino; Cuevas-Bermúdez A; Navarro F.

Advances in Experimental Medicine and Biology 2018

DOI: 10.1007/978-3-030-00737-9_8

 Rpb5 modulates the RNA polymerase II transition from initiation to elongation by influencing Spt5 association and backtracking

Verónica Martínez-Fernández; Ana Isabel Garrido-Godino; María Carmen Mirón-García; Victoria Begley; Antonio Fernández-Pévida; Jesús de la Cruz; Sebastián Chávez; Francisco Navarro

Biochimica et Biophysica Acta - Gene Regulatory Mechanisms 1861(1):1-13 (2018)

DOI:10.1016/j.bbagrm.2017.11.002

Genomic and transcriptomic approaches to the identification of key molecular markers and gene variants on phenol content and composition in olive

Entidad Financiadora: Concesión de ayudas a Proyecto de I+D+i en el marco de los programas estatales de generación de conocimiento y fortalecimiento científico y tecnológico del sistema de I+D+i y de I+D+i orientada a los retos de la sociedad. Convocatoria 2020.Ministerio de Ciencia e Innovación

Referencia: PID2020-115853RR-C33                

Investigador principal: Francisco Luque Vázquez

Año de inicio: 2021

Presupuesto: 160.000,00 €

Homeostasia del RNA en células eucariotas: control de la transcripción global durante el ciclo celular y papel de la Prefoldina-like BUD27

Entidad Financiadora: Concesión de ayudas a Proyecto de I+D+i en el marco de los programas estatales de generación de conocimiento y fortalecimiento científico y tecnológico del sistema de I+D+i y de I+D+i orientada a los retos de la sociedad. Convocatoria 2020.Ministerio de Ciencia e Innovación

Referencia: PID2020-112853GB-C33

Investigador principal: Francisco Navarro Gómez

Año de inicio: 2021

Presupuesto: 120.000,00 €

Contribución de la cochaperona prefoldin-like Bud27 a la formación de los complejos transcripcionales de la RNA polimerasa III, a su actividad transcripcional y a la composición proteica de la cromatina en Saccharomyces cerevisiae

Entidad Financiadora: Ayudas a la I+D+i, en el ámbito del Plan Andaluz de Investigación, Desarrollo e Innovación, PAIDI

Referencia: PY20_00792

Investigador principal: Francisco Navarro Gómez

Año de inicio: 2021

Presupuesto: 60.000,00 €

Influencia de la prefoldina-like URI/Bud27 en la expresión y en los niveles intracelulares de tRNA y su correlación con el cáncer

Entidad Financiadora: Junta de Andalucía-Universidad de Jaén (Proyecto FEDER-UJA)

Referencia: 1260360

Investigador principal: Francisco Navarro Gómez

Año de inicio: 2020

Presupuesto: 108.000,00 €

Olive genome sequencing

Determine and identify all genes and their possible functions

Comparison between varieties

Identification of genes with commercial interest

Verticillium wilt diagnostic kit